S8191
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 | Immunoblotting Whole HeLa cell extracts treated with thymidine were separated on SDS-PAGE and blotted with Rabbit Anti-Survivin (Cat No. S8191). The antibody was developed with Goat Anti-Rabbit IgG, Alkaline Phosphatase (Cat No. A9919) and SIGMA FAST™ BCIP/NBT substrate (Cat. No. B5655). Inhibition with survivin peptide (Lane 2). |
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S8191
Sigma
Anti-Survivin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-BIRC5, Anti-TIAP |
| MDL number: | MFCD02686632 |
Description
| Immunogen | synthetic peptide corresponding to the C-terminus of human survivin (amino acids 122-142). This sequence is highly conserved (>70%) in rat and mouse survivin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 16 kDa |
| species reactivity | rat, human, mouse |
| application(s) | immunoblotting: 1:500 using whole cell extract of human epitheloid carcinoma HeLa cell treated with thymidine. |
| microarray: suitable | |
| Gene Information | human ... BIRC5(332) |
| rat ... Birc5(64041) | |
| mouse ... Birc5(11799) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 2 |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Li, L., et al., Isoflurane preconditioning improves long-term neurologic outcome after hypoxic-ischemic brain injury in neonatal rats. Anesthesiology 107, 963-970, (2007) Abstract |
